3Rs-based procedures have to be used in the field of chemical risk assessment. Thus, we first propose to evaluate developmental neurotoxicity on cell models such as  neural stem cells coming from the subventricular zone. We can thus verify whether chemicals alter relevant cellular mechanisms involved in brain development (survival, migration, differentiation). We also propose models of neuron, astrocyte and microglia primary culture. In case compounds display disturbing activities, we propose in vivo models, compliant with OECD guidelines TG 424 and 426.

We primarily evaluate NT and DNT risks associated to your chemicals in compliance with requirements emanating from OECD guidelines TG 424 and 426. We can assess either all or only one part of these requirements. We can also add relevant outcomes and customize procedures if needed. You can find examples in our publications (see refs 1, 2 and 3 in the footnotes). Feel free to contact us for additional details.

NB PharmaTox assesses efficacy of your candidate molecules on brand new mouse models of CNS disorders, the specific feature of which lying in the fact that related disorder is of developmental & environmental origin, i.e. resulting from exposure to chronic & low dose environmental chemical exposure during the perinatal period of brain development. Therefore, we are able to propose developmental model of autism-like disorders and Schizophrenia-like symptoms (unpublished work). We also offer the possibility to work with genetic model of Schizophrenia.

Thanks to our expertise in both automated (videotracking softwares) and ethologically-relevant measurement, we are able to decipher behavioral defects displayed by your mice after genetic, parmacological or environmental manipulations. To this end, we benefit from a variety of apparatus / assays to make your animals speak . 3R-based procedures can be used to make your experiments more respectul of animal welfare.

Measurement of emotional reactivity, repetitive behavior, and locomotion.

Measurement of emotional reactivity, risk assessment and locomotion.

Measurement of emotional reactivity and risk assessment behavior.

Measurement of mobility, grooming, vertical activity.

Measurement of motor impairments.

Measurement of motor impairments.

Measurement of spatial learning / memory and mental flexibity

Measurement of spatial learning / memory skills, obsessive behavior and working memory.

Measurement of sociability (indirect social interaction) and social cognition (preference for social novelty).

Measurement of sociability, social fear, agressivity.

In vitro measurement of neuroblast migration, neural stem cell differenciation into mature neurons, glial cells and oligodendrocytes.

In vitro measurement of cell survival, activation, ability to produce neuroactive componds (neurotransmitters, kynurenine pathway metabolites etc)

In vitro measurement of cell survival, activation, ability to produce immune mediators or neuroactive compounds (kynurenine pathway metabolites).

In vitro measurement cell survival, activation, ability to produce inflammatory mediators or neuroactive compounds (kynurenine pathway metabolites).